Construction and Clinical Application of Chemiluminescent Immunoassay for Human Serum Ferritin

doi:10.3969/j.issn.1673-1689.2019.03.017

Home > Archive>Volume 38, Issue 3, 2019 >118-123. DOI:10.3969/j.issn.1673-1689.2019.03.017

Construction and Clinical Application of Chemiluminescent Immunoassay for Human Serum Ferritin
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                        10.3969/j.issn.1673-1689.2019.03.017
                    
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To develop a chemiluminescent immunoassay method for detecting serum ferritin with biotin-avidin amplified system and a sandwich way. A pair of antibodies were labeled with biotin and horseradish peroxidase respectively. The 96 well microplate was coated with streptavidin. The serum ferritin standard and international standard was evaluated. 300 clinical serum specimens wer detected with this method and a foreign serum ferritin kit（Roche），the correlation coefficient was 0.97. The linear measurement range of this method was 10~800 ng/mL. The intra-assay and inter-assay coefficients of variation were 3.4~5.5%， and 5.0~7.3%. The cross-reactivity rate with CEA（carcinoembryonic antigen），human albumin，human heart ferritin，and hemoglobin were all less than 1%. The developed method was stable 9 days at 37 ℃. This assay was easily operable，small amount of specimens needed and the reaction was quick，so it was suitable for clinical application.

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SUN Shanshan, LI Xin, SONG Cuicui, YANG Yankun, BAI Zhonghu. Construction and Clinical Application of Chemiluminescent Immunoassay for Human Serum Ferritin[J]. Journal of Food Science and Biotechnology,2019,38(3):118-123.

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Online: June 21,2019
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