Optimal Feeding Timing of Ethanol Fed-Batch Fermentation Based on Yeast Vitality of the Secondary Fermentation

doi:10.3969/j.issn.1673-1689.2019.03.019

Home > Archive>Volume 38, Issue 3, 2019 >130-137. DOI:10.3969/j.issn.1673-1689.2019.03.019

Optimal Feeding Timing of Ethanol Fed-Batch Fermentation Based on Yeast Vitality of the Secondary Fermentation
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                        10.3969/j.issn.1673-1689.2019.03.019
                    
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To explore basic information for ethanol production from sugarcane molasses in fed-batch fermentation，yeast cell vitality of the secondary fermentation was investigated with aim at determining optimal feeding time based on the cell vitality. By batch fermentation at different initial sugar，optimal initial sugar concentration（220 g/L） for fed-batch fermentation was obtained. The secondary fermentations were done by inoculating the same amount of cells from the primary fermentation at 0，6，12，18，24，30，36 h，with emphasis on analyzing cell activity，sugar utilization and ethanol production for determining time point of highest cell vitality in the primary fermentation. By ethanol fed-batch fermentation with feeding timing based on cell vitality，the results showed that the optimum fermentation parameters were observed with feeding at 24 h when the cell vitality was the strongest，namely，the highest ethanol concentration（136.62±1.10） g/L，volumetric productivity（2.53±0.02） g/（L·h） and total sugar fermentation efficiency（83.34%） were achieved，indicating that the cell vitality of yeast based on secondary fermentation can be used as a parameter of feeding time in the ethanol fed-batch fermentation.

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KE Shanhui, WU Shihua, ZHAO Dongling, ZHANG JIAN, HUANG Cuiji. Optimal Feeding Timing of Ethanol Fed-Batch Fermentation Based on Yeast Vitality of the Secondary Fermentation[J]. Journal of Food Science and Biotechnology,2019,38(3):130-137.

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Online: June 21,2019
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