Isolation and Characterization of Siderophore Producing Bacteria for Biocontrol Botryosphaeria dothidea

doi:10.3969/j.issn.1673-1689.2019.03.021

Home > Archive>Volume 38, Issue 3, 2019 >144-151. DOI:10.3969/j.issn.1673-1689.2019.03.021

Isolation and Characterization of Siderophore Producing Bacteria for Biocontrol Botryosphaeria dothidea
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                        10.3969/j.issn.1673-1689.2019.03.021
                    
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Botryosphaeria spp. are important pathogens on a variety of fruit trees causing cankers and gummosis on almond trunks，and resulting in a significant decline in the health development of the planting industry. The strain H47（producing catechol-type siderophore） and the strain H114（producing hydroxamate-type siderophore） were isolated from the honey. The two strains identified as Bacillus amyloliquefaciens by 16S rRNA gene analysis and physiology biochemistry experiment. The strains were cultured with the MM9 medium，and the cell-free supernatants were extracted by organic solvent. The organic phases collected as partially purified siderophore were dried in a vacuum oven. The partially purified siderophore was inoculated along with the B. dothidea Ces. & De Not ACCC 38026 and the inhibition of the mycelial growth was test by vernier caliper. The results indicated that both catechol-type and hydroxamate-type siderophores had antifungal activity to pathogen，toxicity regression equations were y=1.432x+0.578 and y=1.744x+0.063，respectively. Thus these honey isolates had the potential as the bio-control agents，which were useful to inhibit the growth of B. dothidea.

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LI Xunhang, ZHANG Yanzhou, LI Yunya, SUN Meng, GUAN Zhengbing, CAI Yujie, LIAO Xiangru. Isolation and Characterization of Siderophore Producing Bacteria for Biocontrol Botryosphaeria dothidea[J]. Journal of Food Science and Biotechnology,2019,38(3):144-151.

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Online: June 21,2019
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