Isolation，Purification，Structural Characterization and Immunological Activity of Fomitopsis pinicola Polysaccharides

doi:10.3969/j.issn.1673-1689.2019.03.022

Home > Archive>Volume 38, Issue 3, 2019 >152-159. DOI:10.3969/j.issn.1673-1689.2019.03.022

Isolation，Purification，Structural Characterization and Immunological Activity of Fomitopsis pinicola Polysaccharides
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                        10.3969/j.issn.1673-1689.2019.03.022
                    
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Fomitopsis pinicola is a medicinal mushroom and a kind of new resource of food to explore. Two water-soluble crude polysaccharides were extracted using hot water and 5% of NaoH solution.，and then purified FPS by DEAE-Sepharose FF and Sepharose Cl-6B chromatography. Four polysaccharide factions named as FPS1-1，FPS1-2，FPS2-1 and FPS2-2 were isolated and purified from FPS. The result of the MTT test about normal mice spleen lymphocyte proliferation promotion activity indicated that the four polysaccharide factions both have a immunological activity. The effect of FPS1-2 and FPS2-1 are in a dose-dependent manner，and the effect of FPS2-1 was better than others. The HPSEC analysis indicated that the two fractions were all homogeneous polysaccharides and their relative molecular mass were respectively 9.10×103 and 3.02×105. The analysis of monosaccharide composition，infrared spectrum and NMR spectroscopy indicated that FPS1-2 was a heteropolysaccharide，it have α-（1→6）glucosidic bond；and FPS2-1 was a heteropolysaccharide ，it have α-（1→4）glucosidic bond.

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PENG Huiqun, XU Xiaogang, CHEN Zhaoyue, WANG Li, DAI Jun. Isolation，Purification，Structural Characterization and Immunological Activity of Fomitopsis pinicola Polysaccharides[J]. Journal of Food Science and Biotechnology,2019,38(3):152-159.

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