Isolation of a Lactic Acid Bacterium With High Ability of Purine Nucleoside-Decomposing and Evaluation of Its Probiotic Properties

doi:10.3969/j.issn.1673-1689.2019.04.002

Home > Archive>Volume 38, Issue 4, 2019 >8-14. DOI:10.3969/j.issn.1673-1689.2019.04.002

Isolation of a Lactic Acid Bacterium With High Ability of Purine Nucleoside-Decomposing and Evaluation of Its Probiotic Properties
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                        10.3969/j.issn.1673-1689.2019.04.002
                    
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Lactic acid bacteria（LAB） can be used for prevention and auxiliary treatment of hyperuricemia and its complications. By using such way，the detrimental effect of people’s living quality caused by maintaining strict dietary restriction（the conventional treatment of hyperuricemia） could be reduced or eliminated. In this study the purine nucleoside-decomposing ability of LABs were isolated from Chinese sauerkraut bought from local supermarket by HPLC method through the determination of their insosine and guanosine degradation abilities. Among which strain 5-1 showed the best probiotic potential with degradation rate of insosine and guanosine are 89.37% and 93.20% respectively. Based on physiological，biochemical tests and 16S rDNA classification，strain 5-1 was identified as Lactobacillus curvatus. After 4 h treatment under the circumstance of pH 3 or 0.3% bile salt，strain 5-1 could keep a survival level of 107 cfu/mL. Furthermore the great tolerance capacity was shown in the artificial gastrointestinal fluid with food suspension and excellent purine nucleoside-decomposing ability in artificial intestinal juice with food suspension．Therefore，Lb. curvatus 5-1 has a great potential for applications in the development of microbial agents that help to prevent and auxiliarily treat hyperuricemia.

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MA Jumei, ZHAO Weirui, HU Sheng, WANG Kang, HUANG Jun, ZHENG Xiaodong, JIN Zhihua, MEI Lehe. Isolation of a Lactic Acid Bacterium With High Ability of Purine Nucleoside-Decomposing and Evaluation of Its Probiotic Properties[J]. Journal of Food Science and Biotechnology,2019,38(4):8-14.

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