Study on Enzyme Hydrolysis of Waste Activated Sludge Based on Protein Recovery

doi:10.3969/j.issn.1673-1689.2019.04.014

Home > Archive>Volume 38, Issue 4, 2019 >90-96. DOI:10.3969/j.issn.1673-1689.2019.04.014

Study on Enzyme Hydrolysis of Waste Activated Sludge Based on Protein Recovery
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                        10.3969/j.issn.1673-1689.2019.04.014
                    
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Protein recovery from waste activated sludge（WAS） is a kind of new way of sludge reutilization. The purpose of this manuscript is to investigate a combined method of enzymatic hydrolysis with thermal alkali hydrolysis for efficiently dissolving proteins from WAS. The results showed that thermal alkali hydrolysis strongly promoted the dissolution of WAS proteins after combined with enzymatic hydrolysis. Compared with single enzyme hydrolysis，the protein dissolution rates hydrolyzed by lysozyme and papain were increased by 46.84% and 45.56%，respectively. Combined with thermal alkali hydrolysis，the multi-enzymes with alkaline protease and papain got the highest dissolution rate of WAS proteins. With 30 g/L of WAS and 1% of multi-enzymes（alkaline protease∶papain=1∶12），the dissolution of WAS proteins was optimal after WAS was enzyme-hydrolyzed for 2.0 h under pH=7.0 and 55 ℃，and then experienced thermal alkali hydrolysis under pH=12.5 and 90 ℃ for 1.5 h. Accordingly，the soluble protein concentration and dissolution rate were 6 050 mg/L and 84.33%.

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SONG Xiaoli, SHI Zhenghua, LI Xiufen, WANG Xinhua, REN Yueping. Study on Enzyme Hydrolysis of Waste Activated Sludge Based on Protein Recovery[J]. Journal of Food Science and Biotechnology,2019,38(4):90-96.

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