Research of α-Glucosidase Inhibitor From Proteolytic Product of Porphyra yezoensis Chelated With Zinc

doi:10.3969/j.issn.1673-1689.2019.04.021

Home > Archive>Volume 38, Issue 4, 2019 >143-149. DOI:10.3969/j.issn.1673-1689.2019.04.021

Research of α-Glucosidase Inhibitor From Proteolytic Product of Porphyra yezoensis Chelated With Zinc
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                        10.3969/j.issn.1673-1689.2019.04.021
                    
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In order to study the conditions of chelating Znic with α-glucosidase inhibitory active peptides from Porphyra yezoensis enzymatic hydrolysis and evaluate the stability of gastrointestinal digestion The polypeptide，inhibiting the α-glucosidase activity highly，would be gained by the compound enzymolysis technics with using protease of ex- and in- under various treatment factors. Thereafter，the hydrolysate was separated by filtering membrane technique combined ultrafiltration with nanofiltration firstly，and then concentration through rotary evaporator，which the inhibition ratio of α-glucosidase（0.5 mg/mL） could be reached 68% at 1 mg/mL of concentrated polypeptide solution；The optimization factors of chelating reaction involved polypeptide and zinc solution were 1.5 h of reaction time，37 ℃ of temperature，6 mg/ml of polypeptide and pH 4.5 respectively，which the chelating degree of polypeptide and zinc had reached 25.6%， and the inhibition for α-glucosidase had increased to 79.8% after chelation；In addition，the model of gastrointestinal digestion in vitro would be built to evaluate the toleration of the chelate for gastrointestinal digestion by assessing their capacity to inhibit the α-glucosidase activity. The results indicated that the decrease of α-glucosidase activity only had 7% after gastrointestinal digestion under various reaction time and enzyme-substrate ratio；and then reaching 5% approximately after duodenal digestion. Therefore，the chelate of polypeptide and zinc solution was shown better stability of gastrointestinal digestion in this study.

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HU Chunqin, LONG Jing, ZHOU Nandi, TIAN Yaping. Research of α-Glucosidase Inhibitor From Proteolytic Product of Porphyra yezoensis Chelated With Zinc[J]. Journal of Food Science and Biotechnology,2019,38(4):143-149.

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