High-Efficient Preparation of Immobilized Trypsin with Microwave

doi:10.3969/j.issn.1673-1689.2019.05.013

Home > Archive>Volume 38, Issue 5, 2019 >86-93. DOI:10.3969/j.issn.1673-1689.2019.05.013

High-Efficient Preparation of Immobilized Trypsin with Microwave
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                        10.3969/j.issn.1673-1689.2019.05.013
                    
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To find the efficient preparation method of the immobilized trypsin，the microwave-assisted method was applied to improve the efficiency of the covalent enzyme immobilization process. The conditions of preparing immobilized trypsin under microwave irradiation with D151 resins as the carrier were optimized by use of central composite design-response surface method （CCD-RSM）. Through fitting quadratic polynomial regression equations，the optimum immobilization conditions were obtained as follows：adsorption time 1.5 h，glutaraldehyde concentration 1.0%，addition of trypsin 8.2 mg/mL，pH 5.0，crosslinking temperature 29.0 ℃，and crosslinking time 3.5 min. The immobilized trypsin was stable under the condition of 25～45 ℃ as well as pH range was 4.0～5.0 or 9.0～10.0. The immobilized trypsin still could retain 73.1% of its initial activity after reused for 8 cycles. The immobilized trypsin could keep 71.1% of its initial activity with storage at 4 ℃ after 5 weeks. Furthermore，the immobilized trypsin had higher efficiency to hydrolyze casein than free trypsin. In conclusion，the proposed microwave-assisted method is an available and efficient method to produce the immobilized trypsin with excellent catalytic properties.

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LIU Weihua, JIANG Zitao, LI Rong. High-Efficient Preparation of Immobilized Trypsin with Microwave[J]. Journal of Food Science and Biotechnology,2019,38(5):86-93.

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Online: July 26,2019
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