Identification of Adulterated Honey Based on HPLC-ELSD and Partial Least Squares-Discriminant Analysis

doi:10.3969/j.issn.1673-1689.2019.05.021

Home > Archive>Volume 38, Issue 5, 2019 >147-152. DOI:10.3969/j.issn.1673-1689.2019.05.021

Identification of Adulterated Honey Based on HPLC-ELSD and Partial Least Squares-Discriminant Analysis
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                        10.3969/j.issn.1673-1689.2019.05.021
                    
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In order to identify the honey adulteration，this paper present a new high performance liquid chromatography method，coupled with chemometric analysis，used to identification of adulterated honey. The sugar composibion of 33 honey samples obtained from different areas were separated and detected by HPLC-ELSD equipped with Amide column，The method relies on the use of chemometric methods to process chromatograms in order to achieve a better discrimination between authentic and adulterated honeys by partial least squares-discriminant analysis（PLS-DA）. This approach was investigated using honey samples adulterated from 10% to 30% with high fructose syrup and maltose syrup. Good result were obtained in the characterization of authentic and adulterated samples：92.5% of good classification in the training set and 100% in the test set.

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XU Yanchao, CHEN Shangwei, ZHU Song, JI Fubiao, DAI Jun. Identification of Adulterated Honey Based on HPLC-ELSD and Partial Least Squares-Discriminant Analysis[J]. Journal of Food Science and Biotechnology,2019,38(5):147-152.

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Online: July 26,2019
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