Solid-State Fermentation of Trichoderma harzianum for High Yield Spore with Waste Bacteria Stick

doi:10.3969/j.issn.1673-1689.2019.06.003

Home > Archive>Volume 38, Issue 6, 2019 >17-24. DOI:10.3969/j.issn.1673-1689.2019.06.003

Solid-State Fermentation of Trichoderma harzianum for High Yield Spore with Waste Bacteria Stick
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                        10.3969/j.issn.1673-1689.2019.06.003
                    
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The solid-state fermentation of Trichoderma harzianum，in which waste bacteria stick were used as substrate，was optimized by using single-factor test and response surface methodology（RSM）. The up-scaled solid-state fermentation was carried out. Results showed that the optimal conditions were：inoculation 7.5 g/dL（w/w），substrate loading quantity 20 g，natural pH，culture temperature 28 ℃ and fermentation time 144 h. The optimal conditions of response surface analysis method were 57.07%（w/w） initial water content，3.08% starch and 1.99% NaNO3. The theoretical spore yield was 5.65×109 cfu/g compared with the actual yield 5.91×109 cfu/g，which was consistent with the theoretical prediction. The eventual sporulation was 8.20×109 cfu/g at the condition of ventilation by using the 10 L solid-state fermenter.

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WANG Qiaohe, ZHU Li, ZHENG Zhiyong, GAO Minjie, ZHAN Xiaobei. Solid-State Fermentation of Trichoderma harzianum for High Yield Spore with Waste Bacteria Stick[J]. Journal of Food Science and Biotechnology,2019,38(6):17-24.

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Online: January 07,2020
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