Enhancement of Heme Synthesis Pathway in Escherichia coli via a Modular Optimization Strategy

doi:10.3969/j.issn.1673-1689.2019.06.012

Home > Archive>Volume 38, Issue 6, 2019 >86-94. DOI:10.3969/j.issn.1673-1689.2019.06.012

Enhancement of Heme Synthesis Pathway in Escherichia coli via a Modular Optimization Strategy
DOI:
                        10.3969/j.issn.1673-1689.2019.06.012
                    
CSTR:
                        [cstr]
                    
Author:
                        
                        
                    
Affiliation:
Clc Number:Q815
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

Heme，an iron containing prothetic group for oxygen transfer in the body，is a good kind of blood tonic. At present，only few studies on heme biosynthesis have been reported. The regulatory mechanism of heme biosynthesis is still unclear. In the present study，by applying the modular combination strategy，the committed genes hemA and hemL as the first module was overexpressed by the high copy number plasmid pUC19. In parallel，the downstream heme biosynthesis pathway genes were divided into the second（hemB，hemC，hemD and hemE） and third（hemF，hemG，and hemH） modules，and were combinatorially expressed by the plasmids pRSFDuet-2，pCDFDuet-2 and pACYCDuet-2. In comparison，the recombinant strain E. coli D6 achieved the highest heme titer（0.56 μmol/（L·OD）） at 48 h in shake flask cultures. By applying a batch fermentation strategy，the heme production was increased to 0.954 μmol/（L·OD） at 48 h in 3 L fermenter.

Reference

Cited by

Get Citation

WENG Huanjiao, DING Wenwen, SHI Yanan, LI Jianghua, KANG Zhen. Enhancement of Heme Synthesis Pathway in Escherichia coli via a Modular Optimization Strategy[J]. Journal of Food Science and Biotechnology,2019,38(6):86-94.

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:
Revised:
Adopted:
Online: January 07,2020
Published:

Article QR Code

Copy Right：Editorial Board of Journal of Food Science and Biotechnology

Address：No. 1800, Lihu Avenue, Wuxi 214122, Jiangsu Province,China PostCode：214122

Phone：0510-85913526 E-mail：xbbjb@jiangnan.edu.cn

Supported by:Beijing E-Tiller Technology Development Co., Ltd.

Get Citation

Share

Article Metrics

History

Article QR Code

WeChat

Mobile website