Activity of Internal Ribosme Entry Site in mRNA 5′ Untranslation Region of Transcription Factor FOSB

doi:10.3969/j.issn.1673-1689.2019.07.006

Home > Archive>Volume 38, Issue 7, 2019 >41-48. DOI:10.3969/j.issn.1673-1689.2019.07.006

Activity of Internal Ribosme Entry Site in mRNA 5′ Untranslation Region of Transcription Factor FOSB
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                        10.3969/j.issn.1673-1689.2019.07.006
                    
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To explore the possible sequence and function of internal ribosme entry site（IRES） in flanking sequence of FOSB. Using the typical bicistronic reporter system of dual-luciferase，constructing the reporter plasmids containing the 5'UTR of FOSB mRNA and then transfecting them into HEK293 cell and 4 strains of cancer cells. Performing the deletion of FOSB mRNA 5'UTR and stimulating the Bel-7402 cell with anticancer drug，paclitaxel or cis-platinum. The results showed that the FOSB mRNA 5'UTR has IRES activity and the sequences of 160nt between -376 and -217 nt are essential to its IRES function. The FOSB IRES activity can be stimulated in Bel-7402 treated with paclitaxel or cis-platinum，especially under the stimulating of paclitaxel. All these findings provide a new thought and direction for the study of FOSB in cancer prevention and treatment.

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MA Jing, SUN Liuliu, MA Wennan, TAO Yifen, CHEN Yun, ZHU RuiYu, JIN Jian. Activity of Internal Ribosme Entry Site in mRNA 5′ Untranslation Region of Transcription Factor FOSB[J]. Journal of Food Science and Biotechnology,2019,38(7):41-48.
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Online: January 23,2020

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