Identification of Thermostable β-Galactosidase-Producing Bacillus Strain from Honey and Enzymatic Properties

doi:10.3969/j.issn.1673-1689.2019.07.007

Home > Archive>Volume 38, Issue 7, 2019 >49-57. DOI:10.3969/j.issn.1673-1689.2019.07.007

Identification of Thermostable β-Galactosidase-Producing Bacillus Strain from Honey and Enzymatic Properties
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                        10.3969/j.issn.1673-1689.2019.07.007
                    
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A β-galactosidase-producing strain was screened from bacillus species isolatated from honey. The strain was identified as Bacillus licheniformis by an in-depth study and named as Bacillus licheniformis SYBC hb15. β-galactosidase expressed by Bacillus licheniformis SYBC hb15 was purified for the study of enzymatic properties using o-nitrophenyl-β-D-galactoside（ONPG） as substrate. The result showed that the optimal reactive temperature of the β-galactosidase was 60 ℃，and it had high thermal stability when incubating the enzyme at 70 ℃ for 60 min with 65% residual activity. Compared to that of the enzyme from Talaromyces thermophilus，glucose presents less inhibition to the hydrolytic activity of the β-galactosidase. Therefore，the β-galactosidase produced by B. licheniformis SYBC hb15 has good potential in the industrial application because of its high thermostability and resistance to glucose of high levels.

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LI Yun, ZHU Shaohua, GUAN Zhengbing, CAI Yujie, LIAO Xiangru. Identification of Thermostable β-Galactosidase-Producing Bacillus Strain from Honey and Enzymatic Properties[J]. Journal of Food Science and Biotechnology,2019,38(7):49-57.

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