Molecular Engineering of Mycoestrogen-Detoxifying Lactonase ZHD101 to Improve Enzyme Thermostability

doi:10.3969/j.issn.1673-1689.2019.07.010

Home > Archive>Volume 38, Issue 7, 2019 >71-77. DOI:10.3969/j.issn.1673-1689.2019.07.010

Molecular Engineering of Mycoestrogen-Detoxifying Lactonase ZHD101 to Improve Enzyme Thermostability
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                        10.3969/j.issn.1673-1689.2019.07.010
                    
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Abstract:

Lactonase ZHD101 which can degrade ZEN effectively is being widely studied. However，its application in feed industry was limited by low thermostability. In this study，we attempted to improve the thermostability of ZHD101 through structure-based rational design and molecular engineering of disulfide bridges. Specifically，7 mutants（A110C/P196C，S136C/R189C，D143C/P181C，S147C/P181C，D199C/A202C，L200C/A231C，and R204C/G205C） were constructed for disulfide bridge formation. Two mutants，S136C/R189C and D143C/P181C，showed enhanced thermostability. Notably，D143C/P181C mutant，which exhibits similar specific activity as wild type enzyme，showed two-fold increase in thermostability after heated at 50 ℃ for 2 min. A quadruplet mutant（S136C/D143C/P181C/R189C） was subsequently constructed and examined，but it showed lower thermostability comparing to D143C/P181C mutant. This study provides significant benefit for the application of ZHD101 in further commercial utilizations.

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XU Zhongxia, LIU Guizhi, LIU Weidong, GUO Ruiting, LI Huazhong, ZHENG Yingying. Molecular Engineering of Mycoestrogen-Detoxifying Lactonase ZHD101 to Improve Enzyme Thermostability[J]. Journal of Food Science and Biotechnology,2019,38(7):71-77.

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Online: January 23,2020
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