Study on Extraction of Silkworm Oil with Aqueous Ethanol Extraction

doi:10.3969/j.issn.1673-1689.2019.09.012

Home > Archive>Volume 38, Issue 9, 2019 >85-91. DOI:10.3969/j.issn.1673-1689.2019.09.012

Study on Extraction of Silkworm Oil with Aqueous Ethanol Extraction
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                        10.3969/j.issn.1673-1689.2019.09.012
                    
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The silkworm pupae was crushed at dry of freezing state respectively and then observed by scanning electron microscopy（SEM）. Aqueous ethanol was used to extract oil and the effects of ethanol concentration，temperature，pH value and solid-liquid ratio on free oil yield were studied. The fatty acid composition，peroxide value and acid value of silkworm pupae oil extracted under optimal conditions were analyzed. The results show that the oil yield was higher when silkworm pupae was crushed under frozen condition. SEM observation shows that comminution under frozen condition is more effective on disrupting cells，therefore it is more favorable for oil extraction. It was also found that maximum oil yield was obtained as 90.76±0.08% under the conditions that ethanol concentration 40%（v/v），temperature 50 ℃，extraction pH 9，solid-to-liquid ratio 1 g：7 mL for 3 h reaction. GC analysis shows that composition and content of fatty acids in silkworm pupae oil from aqueous ethanol extraction were in substantial agreement with that from n-hexane extraction. Both silkworm oils were composed mainly of palmitic acid，oleic acid and linolenic acid. The peroxide value and acid value of crude silkworm pupae oil obtained by aqueous ethanol method was 0.55 mmol/kg and 0.025 mg/g respectively，which was obviously better than that extracted by n-hexane.

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QIN Lingyu, YANG Ruijin, HUA Xiao, ZHAO Wei, ZHANG Wenbin. Study on Extraction of Silkworm Oil with Aqueous Ethanol Extraction[J]. Journal of Food Science and Biotechnology,2019,38(9):85-91.

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