Efficient Production of Bacillus pumilus γ-Glutamyl Transferase in B. subtilis and Its Application of Biosynthesis of L-Theanine

doi:10.3969/j.issn.1673-1689.2019.10.010

Home > Archive>Volume 38, Issue 10, 2019 >67-71. DOI:10.3969/j.issn.1673-1689.2019.10.010

Efficient Production of Bacillus pumilus γ-Glutamyl Transferase in B. subtilis and Its Application of Biosynthesis of L-Theanine
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                        10.3969/j.issn.1673-1689.2019.10.010
                    
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L-theanine，is a nonproteic amino acid which recently has an increasing demand as it is applied in food and pharmaceutical industries. In this study，a GRAS（Generally Recognized As Safe） strain Bacillus subtilis 168 was used as the host to produce a novel γ-glutamyl transferase（GGT） from B. pumilus ML413. The enzyme produced was applied to L-theanine synthesis. Subsequently，glutamine conversion efficiency with the GGT was optimized. Finally，for the improved L-theanine production，a fed-batch strategy was introduced and the maximum titer of L-theanine reached 50.8 g/L at 16 h. This titer could be comparable to the efficient but toxic GGT producer recombinant E.coli.

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KOMERA Irene, YANG Taowei, ZHANG Xian, RAO Zhiming, XU Meijuan. Efficient Production of Bacillus pumilus γ-Glutamyl Transferase in B. subtilis and Its Application of Biosynthesis of L-Theanine[J]. Journal of Food Science and Biotechnology,2019,38(10):67-71.

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