Effects of RNAi Targeting opaque2 Gene Expression on the Kafirin Content in Sorghum

doi:10.3969/j.issn.1673-1689.2019.10.011

Home > Archive>Volume 38, Issue 10, 2019 >72-78. DOI:10.3969/j.issn.1673-1689.2019.10.011

Effects of RNAi Targeting opaque2 Gene Expression on the Kafirin Content in Sorghum
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                        10.3969/j.issn.1673-1689.2019.10.011
                    
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Abstract:

In order to explore the effect of silencing opaque2 gene on the kafirin content in sorghum，the suppressed expression vector of opaque2 gene is constructed by RNAi technology，and then transformed to young embryos by Agrobacterium tumefaciens-mediated transformation system. The kafirin content in these transgenic seeds is determined and indentified by SDS-PAGE，and also the changes of protein bodies in these endosperm cells are observed by transmission electron microscope. The results show that the kafirin content of four fifths of transgenic plants is significantly lower than that of the wild type（P<0.05）. Those of the protein bands between the wild type and all transgenic plants are similar. The starch granules in the endosperm cells of the wild type arranged closely and most of them are fused into large blocks，and large numbers of protein bodies are indicated with large volumes. While the starch granules in the transgenic plants arrayed loosely and indicated many gaps in the endosperm cells，and while those numbers and volumes of the protein bodies are less and smaller than the wild type，respectively. In conclusion，opaque2 gene silencing induced changes in number and volume of protein bodies in endosperm cells，which additionally affects the internal structure of endosperm cells and the kafirin content in sorghum.

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CAI Xinyue, LI Zhijiang, NIU Jiangshuai, BAO Dan, SU Chen, SUN Yimo, DAI Lingyan. Effects of RNAi Targeting opaque2 Gene Expression on the Kafirin Content in Sorghum[J]. Journal of Food Science and Biotechnology,2019,38(10):72-78.

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