Construction and Expression of a Recombinant Bacillus subtilis Producing Levansucrase

doi:10.3969/j.issn.1673-1689.2019.10.012

Home > Archive>Volume 38, Issue 10, 2019 >79-86. DOI:10.3969/j.issn.1673-1689.2019.10.012

Construction and Expression of a Recombinant Bacillus subtilis Producing Levansucrase
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                        10.3969/j.issn.1673-1689.2019.10.012
                    
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Lactosucrose is a rare trisaccharide with prebiotic effect，which can be formed from lactose and sucrose by levansucrase. In this study，levansucrase expression was efficiently carried out by comparing two different promoters and making them tandem. Six different strains with different promoters had been successfully constructed，including WB-PH，WB-P，WB-H，1A-PH，1A-P，1A-H. Amongst them，WB-PH exhibited a higher production level. The effect of growing condition on WB-PH was studied. Using soy peptone as nitrogen source and a higher dissolved oxygen level can enhance levansucrase expression. The enzyme activity reached 108.34 U/mL，which was 25.92 times higher than it before optimization.

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SUN Weiqin, MU Wanmeng, ZHANG Tao, JIANG Bo. Construction and Expression of a Recombinant Bacillus subtilis Producing Levansucrase[J]. Journal of Food Science and Biotechnology,2019,38(10):79-86.

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