Studies on Immobilization of Neutral Protease from Bacillus subtilis

doi:10.3969/j.issn.1673-1689.2019.10.014

Home > Archive>Volume 38, Issue 10, 2019 >98-104. DOI:10.3969/j.issn.1673-1689.2019.10.014

Studies on Immobilization of Neutral Protease from Bacillus subtilis
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                        10.3969/j.issn.1673-1689.2019.10.014
                    
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Chlorhexidine was modified on carrier by cross-linking protocol and the effects of modified carriers on neutral proteases were studied. The reaction conditions were optimized. The additive amount of Chlorhexidine was 0.095 mmol；the activate time of resins was 1 h；the molar ratio of carboxyl to EDC was 1∶3；the addition of neutral protease was 600 U and the addition of EDC was 13.7 mg on the process of activating enzymes. The maximal neutral protease activity was 398 U/g resin. The neutral protease was cross-linked to the affinity carrier through the Fourier transform infrared detection. TheKm values of immobilized，free and randomized immobilized neutral protease were 2.9，1.7 and 7.1 mg/mL，respectively.

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ZHANG Yujun, CAO Hui, XU Fei, YUAN Min, YE Tai, YU Jinsong. Studies on Immobilization of Neutral Protease from Bacillus subtilis[J]. Journal of Food Science and Biotechnology,2019,38(10):98-104.

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