Construction of Lower Optimum pH Mutants of Bacillus flexus β-Amylase and Its Application in Maltose Production

doi:10.3969/j.issn.1673-1689.2019.10.015

Home > Archive>Volume 38, Issue 10, 2019 >105-110. DOI:10.3969/j.issn.1673-1689.2019.10.015

Construction of Lower Optimum pH Mutants of Bacillus flexus β-Amylase and Its Application in Maltose Production
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                        10.3969/j.issn.1673-1689.2019.10.015
                    
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Beta-amylase and other starch hydrolases were complexed to produce high purity maltose syrup in industry. Compared with plant β-amylase，the microbial β-amylase has the advantages of high purity，little limitation with the raw materials，and the suitability for large-scale production. However，the optimum pH of the microbial β-amylase is 6.0~8.0，which is difficult to complex with other starch hydrolases. In the pvious study，the optimum pH of Bacillus flexus β-amylase was 7.0. In the present study，three B.flexus β-amylse mutants T47K，Y164K，L396K were constructed and the optimal pH were changed from 7.0 to 6.0，4.5 and 5.5 respectively. At the same time，the optimum temperature of L396K was increased from 50 ℃ to 60 ℃. The production conditions of maltose using L396K β-amylase was also optimized. When using potato starch（10% w/v） as the substrate under the condition of pH 5.5 and temperature 60 ℃，the yield of maltose reached 80.2%，which satisfied the production requirement of high purity maltose syrup.

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QI Xuhui, WU Jing, WANG Lei, CHEN Sheng. Construction of Lower Optimum pH Mutants of Bacillus flexus β-Amylase and Its Application in Maltose Production[J]. Journal of Food Science and Biotechnology,2019,38(10):105-110.

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