Effects of FKS Family Genes on the Ability of Saccharomyces cerevisiae Stress Resistance

doi:10.3969/j.issn.1673-1689.2019.10.018

Home > Archive>Volume 38, Issue 10, 2019 >126-134. DOI:10.3969/j.issn.1673-1689.2019.10.018

Effects of FKS Family Genes on the Ability of Saccharomyces cerevisiae Stress Resistance
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                        10.3969/j.issn.1673-1689.2019.10.018
                    
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TheFKS family genes which encoded 1，3-β-glucan synthase are important to maintain the integrity of Saccharomyces cerevisiae cell wall. To illustrate the functions of FKSfamily genes on the ability of S. cerevisiae to tolerate the environmental stresses and ethanol production，theFKS1，FKS2，and FKSFKS3 knocked out strains were constructed by homologous recombination，then the physiological properties of wile-type strain and mutant strains were measured and compared. The results showed that the content of 1，3-β-glucan in cell wall of the FKS1 knocked out strain was 60% lower compared to the wild type. The knockout of FKS1 gene also weakened the abilities of growth，stress resistance and ethanol production for S. cerevisiae. Furthermore，the growth and stress resistance abilities of FKS3 gene knocked out strain were similar to those of the wild-type strain，however its abilities to tolerate the fermentation environment and to produce ethanol were better than those of the wild-type strain. Therefore，the FKS1 gene was important for S. cerevisiae to keep the cells activity and to resist the environmental stresses while the FKS3 gene negatively influenced the ability of S. cerevisiae to resist outer stresses.

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YANG Ge, WANG Jinjing, LI Jia, ZHENG Feiyun, LIU Chunfeng, LI Yongxian, NIU Chengtuo, LI Qi. Effects of FKS Family Genes on the Ability of Saccharomyces cerevisiae Stress Resistance[J]. Journal of Food Science and Biotechnology,2019,38(10):126-134.

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