Ameliorative Effects of Fraction 1 from the Flower of Edgeworthia gardneri（Wall.） Meisn on Insulin Resistance of C2C12 Cells

doi:10.3969/j.issn.1673-1689.2019.11.008

Home > Archive>Volume 38, Issue 11, 2019 >55-62. DOI:10.3969/j.issn.1673-1689.2019.11.008

Ameliorative Effects of Fraction 1 from the Flower of Edgeworthia gardneri（Wall.） Meisn on Insulin Resistance of C2C12 Cells
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                        10.3969/j.issn.1673-1689.2019.11.008
                    
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To investigate the ameliorative effects of n-Hexane extract Fraction 1（Fr.1） from the flower of Edgeworthia gardneri（Wall. ） Meisn on insulin resistance（IR），we established IR cell model of C2C12 by using palmitic acid（PA）. Glucose consumption，glucose uptake，relative mRNA and protein expression were detected by the glucose oxidase method，2-NBDG uptake，qRT-PCR and western blot analyses. 0.75 mmol/L PA and 16 hours were the optimal concentration and time to induce IR in C2C12 cells. In the range of non-cytotoxic concentration（12.5~100 μg/mL），Fr.1 promoted glucose consumption and uptake in C2C12/IR. Fr.1 also significantly upregulated Insulin receptors（IRs），Insulin receptor substrate-1（IRS-1），Glut4，PI3K and AMPKα mRNA expression and increased p-IRs，p-IRS，Glut4，p-AMPKα，p-ACC and p-AKT protein expression. Fr.1 from the flower of E. gardneri ameliorated IR through modulating IRs/IRS-1/PI3K/Akt/Glut4 and AMPK/ACC signaling pathways in C2C12 cells.

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MENG Zhaomin, GENG Yan, LI Heng, XU Hongyu, XU Zhenghong, ZHAO Hui, LIU Min, SHI Jinsong. Ameliorative Effects of Fraction 1 from the Flower of Edgeworthia gardneri（Wall.） Meisn on Insulin Resistance of C2C12 Cells[J]. Journal of Food Science and Biotechnology,2019,38(11):55-62.

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