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Home > Archive>Volume 38, Issue 11, 2019 >78-84. DOI:10.3969/j.issn.1673-1689.2019.11.011
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Isolation and Purification of Exopolysaccharide from Bacillus mucilaginosus SM-01
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    Abstract:

    Since the broth of Bacillus mucilaginosus SM-01 was of high viscosity and hard to be separated，the diatomite sorption-filtration method was applied to improve the extraction efficiency of exopolysaccharide by optimizing the process and ultrafiltration conditions. The bacteria and protein of the broth can be removed facilely by filtration at room temperature under the optimal conditions included：the addition of NaCl is 3 g/dL，the dilution ratio of the broth is 3 times，and the amount of diatomite is 10 g/L. The filtrate can be purified by ultrafiltration（relative molecular-weight cut-off of 200 000） at 0.1 MPa. The recovery of exopolysaccharide was up to 73.2%.

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YU Li, FU Haitian, PENG Mengxia, DENG Chao, CHEN Jinghua. Isolation and Purification of Exopolysaccharide from Bacillus mucilaginosus SM-01[J]. Journal of Food Science and Biotechnology,2019,38(11):78-84.

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  • Online: January 10,2020
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