Effects of Spray Drying on Metabolic Enzyme Activity and Culturability of Lactobacillus delbrueckii

doi:10.3969/j.issn.1673-1689.2019.11.016

Home > Archive>Volume 38, Issue 11, 2019 >115-121. DOI:10.3969/j.issn.1673-1689.2019.11.016

Effects of Spray Drying on Metabolic Enzyme Activity and Culturability of Lactobacillus delbrueckii
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                        10.3969/j.issn.1673-1689.2019.11.016
                    
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In order to improve the culturability of lactic acid bacteria during spray drying ，taking Lactobacillus delbrueckii（ATx） as study objects，culturability of lactic acid bacteria have been investigated after heat stress. And the effects of spray drying on the activities of hexokinase，lactate dehydrogenase，cell membrane ATPase，cell viability and acid-producting capacity have been investigated in four kinds of protective agent（20% skim milk（SM），12% skim milk +8% synanthrin（ SMS），12%skim milk +8% trehalose（SMT），12% skim milk+4% synanthrin +4% trehalose（SMST））. The results showed that the optimal heat stress condition of ATx was（50 ℃，15 min），and the number of viable cells only decreased by 0.107 lg CFU / mL（P≥ 0.05）. It was worth mentioning that after spray drying，the activities of ATPase，carbohydrolase and lactate dehydrogenase were significantly decreased（P <0.05），but all were still higher than the control group. Four kinds of bacterial powderin the 12% skim milk had a good acid-producting capacity and curd time was 8-10 h，these indicated that spray drying could damage the cell membrane and the key enzyme of glycometabolism in ATx. In order to improve the culture culturability ，we could add protective agent to improve the resistance of cells to unfavorable environment in spray drying.

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JIANG Aiting, LI Baokun, JIN Dan, QIAO Chuanli, YANG Jie, ZHAO Lili. Effects of Spray Drying on Metabolic Enzyme Activity and Culturability of Lactobacillus delbrueckii[J]. Journal of Food Science and Biotechnology,2019,38(11):115-121.

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