Expression and Bioinformatic Analysis of an L-Lactate Dehydrogenase（L-LcLDH2） for the Asymmetric Reduction of Phenylpyruvic Acid

doi:10.3969/j.issn.1673-1689.2019.12.004

Home > Archive>Volume 38, Issue 12, 2019 >25-30. DOI:10.3969/j.issn.1673-1689.2019.12.004

Expression and Bioinformatic Analysis of an L-Lactate Dehydrogenase（L-LcLDH2） for the Asymmetric Reduction of Phenylpyruvic Acid
DOI:
                        10.3969/j.issn.1673-1689.2019.12.004
                    
CSTR:
                        [cstr]
                    
Author:
                        
                        
                    
Affiliation:
Clc Number:Q786
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

Using the genomic DNA from Lactobacillus casei CICIM B1192 as the templet，we amplified the coding gene Lcldh2 by PCR technique，which encodes an L. casei L-lactate dehydrogenase（L-LcLDH2）. Then，Lcldh2 was successfully expressed in E. coli BL21（DE3）mediated by an expression vector pET-28a（+）. Using PPA as the substrate，L-LcLDH2 displayed the enzymeactivity of 47 U/mg，by which the asymmetric reduction of the substrate PPA afforded L-PLA with enantiomeric excess（eep） of more than 99%. Bioinformatic prediction revealed that Lcldh2 is 906 bp in length，encoding 301 amino acids. Theoretical relative molecular mass and isoelectric point of L-LcLDH2 is 32 585 and 5.5，respectively. L-LcLDH2 is a stable cytoplasmic protein without signal peptide and has a highly conserved sequence GXGXXG. The acquisition of L-LcLDH2 has provided a new biocatalyst for the preparation ofhighly optically pureL-phenyllactic acid.

Reference

Cited by

Get Citation

LI Xueqing, YUAN Fengjiao, LIU Yan, LI Jianfang, WU Minchen. Expression and Bioinformatic Analysis of an L-Lactate Dehydrogenase（L-LcLDH2） for the Asymmetric Reduction of Phenylpyruvic Acid[J]. Journal of Food Science and Biotechnology,2019,38(12):25-30.

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:
Revised:
Adopted:
Online: April 07,2020
Published:

Article QR Code

Copy Right：Editorial Board of Journal of Food Science and Biotechnology

Address：No. 1800, Lihu Avenue, Wuxi 214122, Jiangsu Province,China PostCode：214122

Phone：0510-85913526 E-mail：xbbjb@jiangnan.edu.cn

Supported by:Beijing E-Tiller Technology Development Co., Ltd.

Get Citation

Share

Article Metrics

History

Article QR Code

WeChat

Mobile website