Enzymatic Synthesis and Application of Phytanol-Phosphate Mannose

doi:10.3969/j.issn.1673-1689.2020.03.007

Home > Archive>Volume 39, Issue 3, 2020 >49-55. DOI:10.3969/j.issn.1673-1689.2020.03.007

Enzymatic Synthesis and Application of Phytanol-Phosphate Mannose
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                        10.3969/j.issn.1673-1689.2020.03.007
                    
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Mannosylation in the endoplasmic reticulum（ER）lumen is common to four glycosylation pathways，and involves N-linked glycosylation，glycosylphosphatidylinositol（GPI)-anchor and protein O- and C-mannosylation. Thus，mannosylation in the ER lumen is vital for eukaryotes. The donor for mannosylation in the ER lumen is dolichol-phosphate-mannose（Dol-P-Man)，thus the synthesis of Dol-P-Man is important for understanding different kinds of glycosylation modification. Dol-P-Man synthase catalyzes the transfer of mannose from GDP-mannose（GDP-Man）to dolichol-phosphate（Dol-P） to produce Dol-P-Man. This study expressed yeast Dol-P-Man synthase（Dpm1）in Escherichia coli，and purified it. The conserved DXD motif in Dpm1 playsed crucial roles for these activities. We used phytanol with a short lipid chain to substitute for its dolichol homologue with a long lipid chain，and synthesized phytanol-phosphate mannose（Phy-P-Man）using purified Dpm1. Furthermore，Phy-P-Man could be used as a substrate for Alg3 for N-glycosylation research.

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LI Shengtao, WANG Ning, GAO Xiaodong. Enzymatic Synthesis and Application of Phytanol-Phosphate Mannose[J]. Journal of Food Science and Biotechnology,2020,39(3):49-55.

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