Effect of Amino Acid Transporter System Modification on Extracellular L-methionine Accumulation in Escherichia coli

doi:10.3969/j.issn.1673-1689.2020.03.008

Home > Archive>Volume 39, Issue 3, 2020 >56-64. DOI:10.3969/j.issn.1673-1689.2020.03.008

Effect of Amino Acid Transporter System Modification on Extracellular L-methionine Accumulation in  Escherichia coli
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                        10.3969/j.issn.1673-1689.2020.03.008
                    
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For the research of amino acid transporter system，it is important to construct a rational amino acid production strain. In this study， Escherichia coli 3110 mutants with MetD methionine absorption system pertinent genes metI，metN or metQ deleted were constructed to confirm the main factor in extracellular methionine absorption. Then genes yjeH and yeaS of methionine secretion system were overexpressed by plasmids and genomic integration to investigate the comprehensive effects on extracellular methionine accumulation and biomass. Compared with wild type strain W3110，the methionine uptake capacity of metI gene damaged strain decreased 16.7%. Combining yjeH and yeaS overexpression through episomal plasmid，the methionine accumulation could reach 0.21 g/L and 0.18 g/L，and the concentration increased 128% and 38.9% after yjeH and yeaS overexpression through genomic integration，reached 0.48 g/L and 0.25 g/L，respectively. In conclusion，to improve amino acid secretion and absorption simultaneously is effective for extracellular methionine accumulation and this mode can regarded as a strategy for constructing rational amino acid production strains.

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DONG Wei, LI Youran, ZHANG Liang, DING Zhongyang, SHI Guiyang. Effect of Amino Acid Transporter System Modification on Extracellular L-methionine Accumulation in Escherichia coli[J]. Journal of Food Science and Biotechnology,2020,39(3):56-64.

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Online: May 21,2020
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