Preparation of Chitosan Oligosaccharides by Enzymatic Hydrolysis and Evaluation of Antitumor Activities

doi:10.3969/j.issn. 1673-1689.2021.06.012

Home > Archive>Volume 40, Issue 6, 2021 >93-99. DOI:10.3969/j.issn. 1673-1689.2021.06.012

Preparation of Chitosan Oligosaccharides by Enzymatic Hydrolysis and Evaluation of Antitumor Activities
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                        10.3969/j.issn. 1673-1689.2021.06.012
                    
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To further analyze the antitumor activities and mechanism of chitosan oligosaccharides,enzymatic hydrolysis method was applied to obtain chitosan oligosaccharides with clear composition. The optimized enzymatic hydrolysis conditions were 4 h of reaction time and 120 U/g for the ratio of enzyme and substrate dosage. Chitosan oligosaccharides with degree of polymerization 2 to 4 were obtained by ethanol precipitation, ultrafiltration and nanofiltration. Comparative evaluation of inhibitory effect on three tumor cell lines was conducted with chitosan oligosaccharides and its combination with doxorubicin. The most significant effect was observed on the cell viability of human breast cancer MDA-MB-231 with 20% reduction. Cell migration assay and confocal laser scanning microscopy (CLSM) were used for mechanism analysis, which showed that chitosan oligosaccharides could inhibit cell migration and facilitate doxorubicin to enter the nucleusin MDA-MB-231 cells. The results implied chitosan oligosaccharides could enhance the sensitivity of MDA-MB-231 cells to doxorubicin.

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JI Ke, LI Heng, GONG Jinsong, GENG Yan, DING Zhenzhong, JIANG Min, XU Zhenghong, SHI Jinsong. Preparation of Chitosan Oligosaccharides by Enzymatic Hydrolysis and Evaluation of Antitumor Activities[J]. Journal of Food Science and Biotechnology,2021,40(6):93-99.

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Online: July 26,2021
Published: June 25,2021

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