Abstract:The preparation of pancreatic lipase-inhibiting peptide from ovalbumin(PLIPA) was studied by response surface method, and the stability of PLIPA was studied by simulated gastrointestinal digestion. C57BL/6J mice obesity model was successfully established after feeding high fat diet for 40 days. After 28 d of intragastric administration of PLIPA, the mice were killed and the related indexes were detected to study the control effect of PLIPA on body weight and body shape of nutritional obese mice. The results showed that when the concentration of ovalbumin and alkaline protease were 80 g/L and 2 240 U/g, respectively, the highest inhibitory rate of PLIPA was (56.06±2.64)% after hydrolyzed at 55 ℃, pH 9.0 for 4 h. After digestion of simulated gastric solution and simulated gastric and intestinal solution, the inhibitory rate of PLIPA per milligram of pancreatic lipase were 0.53% and 0.46%, respectively. The overall inhibition rate was still high and the stability was good. The body weight and Lee's index of PLIPA complex group were significantly lower than those of model group (P<0.05). The indexes of abdominal fat coefficient, triglycerides(TG), total cholesterol (TC), and low-density lipoprotein cholesterol(LDL-C) and high-density lipoprotein cholesterol(HDL-C) in the treatment groups were lower than those in model group, indicating that PLIPA could reduce body weight and fat in obese mice, and PLIPA complex group showed the best effect on controlling body weight and body shape.
