Abstract:The effect of Fu brick tea on hepatic lipid synthesis and oxidative stressin jury in APOE-/- mice with non-alcoholic fatty liver was investigated in this study. Fifty-five male APOE-/-mice aged 2 months were continuously fed with high fat diet for 13 week. Five of them were randomly selected for HE staining to estimate the successful development of animal model.The remaining fifty APOE-/- mice were randomly divided into model group,atorvastatin group,and high-, medium- and low-dose groups of Fu brick tea after successful modeling. All of APOE-/- mice were respectively intervened with high fat diet through gavage for 8 weeks. At the same time, 2-month-old C57 mice 2 were selected as the control group and fed with normal diet for 13 weeks and then given saline for 8 weeks. At the end of the experiment, the liver tissues of mice were taken for liver function, oxidative stress, HE staining and RT-qPCR. Pathological morphology showed that the degree of liver steatosis in Fu brick tea of high- and medium-dose groups were lighter than that in the model group. Compared with the control group, the activity of GSH-PX and SOD in the liver of model group decreased, while the content of MDA, the activity of ALT and AST, NAS score, the gene expression of SCD-1、PPAR-γ and HMGCR increased (P<0.05). Compared with the model group, the content of MDA, the activity of ALT and AST, NAS score, the gene expression of SCD-1, PPAR-γ and HMGCR were decreased in different Fu brick tea dose groups, while the GSH-PX and SOD activity increased. Fu brick tea can reverse the early NAFLD by inhibiting hepatic lipid synthesis and reducing hepatic oxidative stress damage.
