Engineering Plasma Membrane to Promote Enzyme Assembly and N-Acetylglucosamine Synthesis

doi:10.3969/j.issn.1673-1689.2021.10.002

Home > Archive>Volume 40, Issue 10, 2021 >7-15. DOI:10.3969/j.issn.1673-1689.2021.10.002

Engineering Plasma Membrane to Promote Enzyme Assembly and N-Acetylglucosamine Synthesis
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                        10.3969/j.issn.1673-1689.2021.10.002
                    
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Functional membrane microdomains(FMMs) can be used as the endogenous spatial scaffolds for pathway enzyme assembly. Moderately increasing the proportion of FMMs in plasma membrane can significantly increase the synthesized product yield. However, the cell growth and product synthesis were both inhibited after further modification of FMMs. Based on the previous research of our group, the rational modification of plasma membrane was carried out to alleviate the effects of excessive FMMs modification on cells. First, the plasma membrane was rationally engineered by overexpression of PlsX, PlsY and PlsC. Then, taking the synthesis of N-acetylglucosamine(GlcNAc) by Bacillus subtilis as an example, it was found that GlcNAc titer in the plasma lemma-modified strains was （5.18±0.16） g/L, which was almost 41.9% higher than that of the control strain. In addition, cell membrane modification also reduced the adverse effect of FMMs over modification on the growth of the strain.

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ZHANG Cheng, LYU Xueqin, JIN Ke, LI Jianghua, DU Guocheng, LIU Long. Engineering Plasma Membrane to Promote Enzyme Assembly and N-Acetylglucosamine Synthesis[J]. Journal of Food Science and Biotechnology,2021,40(10):7-15.

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Online: October 27,2021
Published: October 25,2021

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