Inhibitory Effects of Astragalus Polysaccharideon Activity of Xanthine Oxidase

doi:10.3969/j.issn.1673-1689.2021.10.003

Home > Archive>Volume 40, Issue 10, 2021 >16-20. DOI:10.3969/j.issn.1673-1689.2021.10.003

Inhibitory Effects of Astragalus Polysaccharideon Activity of Xanthine Oxidase
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                        10.3969/j.issn.1673-1689.2021.10.003
                    
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Xanthine oxidase is a key enzyme in uric acid formation, which can effectively reduce the content of uric acid in vivo by inhibiting the activity of xanthine oxidase. To explore the inhibitory effects of Astragalus polysaccharide on xanthine oxidase, the variation of uric acid content of reaction system per unit time was determined by UV-Spectrophotometry, and the inhibition ratio of Astragalus polysaccharide to xanthine oxidase was calculated. The semi-inhibitory concentration IC₅₀ was also obtained. The inhibition type of Astragalus polysaccharide on xanthine oxidase was determined by the Lineweaver-Burk plot, and a Dixon plot was used to obtain the inhibition kinetic parameter(K_i). The inhibitory effects of Astragalus polysaccharide combining allopurinol were explored by the toxicological studies. Astragalus polysaccharide could inhibit the activity of xanthine oxidase, and the IC₅₀ was 1.37 mg/mL. The kinetic analysis showed that Astragalus polysaccharide had competitive and reversible inhibitory effect on xanthine oxidase, with an inhibition constant (K_i) of 0.59 mg/mL. There was antagonism effect when Astragalus polysaccharide was combined with allopurinol. Astragalus polysaccharide could inhibit the activity of xanthine oxidase.

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LI Jing, JIANG Lili, LI Muxuan, DENG Feng, XU Songyao, XUE Xin, HU Ruiping, XUE Huiting. Inhibitory Effects of Astragalus Polysaccharideon Activity of Xanthine Oxidase[J]. Journal of Food Science and Biotechnology,2021,40(10):16-20.

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Online: October 27,2021
Published: October 25,2021

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