Metabolic Engineering of Escherichia coli for Synthesis of Adipic Acid from Fatty Acids

doi:10.3969/j.issn. 1673-1689.2022.03.003

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Metabolic Engineering of Escherichia coli for Synthesis of Adipic Acid from Fatty Acids
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                        10.3969/j.issn. 1673-1689.2022.03.003
                    
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Affiliation:(1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 2. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; 3.International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China)
Clc Number:Q 936
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Abstract:

Adipic acid, as the monomer for manufacturing polymers such as nylon, fiber and plastics, has broad applications in food, pharmaceutical, plastics, and chemical industries. At present, low-level yield of adipic acid generated from food feedstocks was mainly due to the carbon atom loss. To tackle this issue, an artificial adipic acid biosynthesis pathway for obtaining the highest theoretical adipic acid yield from palmitic acid was designed and constructed. Then, cell utilization for fatty acids was improved by enhancing endogenous β -oxidation pathway and introducing heterogenous ω -oxidation pathway. Furthermore, various strategies of system metabolic engineering were used for further optimizing adipic acid biosynthesis pathway. Finally, the optimal recombinant strain Escherichia coli AA0304 produced 1.32 g/L adipic acid by optimizing fermentation conditions. Therefore, this synthetic platform could lay a good foundation for the production of high-value chemicals from waste oleaginous feedstocks.

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LI Yang, YAN Yanan, ZHAO Chunlei, GUO Liang, GAO Cong, LIU Liming, CHEN Xiulai. Metabolic Engineering of Escherichia coli for Synthesis of Adipic Acid from Fatty Acids[J]. Journal of Food Science and Biotechnology,2022,41(3).

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Online: April 19,2022
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