Glycogen as Novel Gene Vector

doi:10.3969/j.issn.1673-1689.2022.05.005

Home > Archive>Volume 41, Issue 5, 2022 >38-45. DOI:10.3969/j.issn.1673-1689.2022.05.005

Glycogen as Novel Gene Vector
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                        10.3969/j.issn.1673-1689.2022.05.005
                    
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This study aims to explore the feasibility and effectiveness of functionalized glycogen as a gene vector. Aminated glycogen (NH₂-Gly) was synthesized through functionalization of glycogen with diethylenetriamine. The structure of NH₂-Gly was characterized by infrared spectra. The encapsulation and protection of pEGFP by NH₂-Gly was studied by gel retardation assay. The biocompatibility of NH₂-Gly was investigated by MTT and hemolysis assay. The transfection efficiency of NH₂-Gly/pEGFP in NIH 3T3 cells was analyzed by confocal laser scanning microscope and flow cytometry. The results showed that the prepared NH₂-Gly was uniformly dispersed with a particle size of about 80 nm and a significant positive charge. Compared with PEI 25 k, NH₂-Gly had good biocompatibility. NH₂-Gly could effectively encapsulate pEGFP and protect it from degradation by nuclease. NH₂-Gly significantly increased the transfection efficiency of pEGFP in NIH 3T3 cells, which was almost equivalent to that of PEI 25 k. Therefore, glycogen is expected to be a safe and efficient gene vector.

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DENG Shuhao, LAI Li, WANG Zhiqing, ZHANG Huijie, CHEN Jinghua. Glycogen as Novel Gene Vector[J]. Journal of Food Science and Biotechnology,2022,41(5):38-45.

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Online: June 03,2022
Published: May 25,2022

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