Abstract:As the main by-products of coffee production, coffee cherry husks are rich in polyphenols. However, its comprehensive development and utilization are limited. In this study, polyphenols in coffee cherry husks were extracted, purified and characterized. The antioxidant activity was determined. The inflammatory model of Caco-2 cell induced by lipopolysacchride (LPS) was established to explore the anti-inflammatory properties of polyphenols. The results showed that the purification effect of LX-17 macroporous resin was excellent. The main components of purified coffee cherry husks polyphenols were 3, 4-dihydroxybenzoic acid (15.78 μg/mg), chlorogenic acid (37.85 μg/mg) and alkaloid caffeine (55.82 μg/mg). The antioxidant assay showed that the extracted polyphenols exhibited good antioxidant activity and showed strong scavenging activity on DPPH, ABTS+ and hydroxyl free radicals. In the inflammatory cell model, 5~200 μg/mL of purified polyphenols could inhibit the expression of proinflammatory factors (IL-6, IL-10, IL-1β and TNF-α) induced by LPS. In conclusion, this study provided a good theoretical basis for the extraction, purification and comprehensive utilization of polyphenols from coffee cherry husks.
