Abstract:The metabolites of Huang Guanyin oolong tea produced in Yunxiao and Wuyishan were analyzed by widely targeted metabolomics and sensory evaluation to investigate the quality differences of Huang Guanyin oolong tea from different areas. The results showed that a total of 820 metabolites from 11 classes were identified in Huang Guanyin oolong tea from the two areas. The different metabolic components identified from the first-grade oolong tea were 63, and 120 for the second-grade oolong tea, accounting for 7.68% and 14.63% of the total metabolites, respectively. The KEGG metabolic pathway analysis showed that three highest enrichment pathways of the first-grade Huang Guanyin oolong teas from two areas were related to the flavonoid differential metabolites, and two highest enrichment pathways of the second-grade Huang Guanyin oolong teas from two areas were related to the differential metabolites of amino acids and their derivatives. According to the comparison of flavor components in Huang Guanyin oolong teas from different areas, the content of differential metabolites including quercetin-3-O-sambubioside, kaempferol-3-O-rhamnoside, apigenin-7-O-rutinoside, L-proline, L-asparagine and γ-aminobutyric acid in the first-grade Huang Guanyin oolong tea from two areas were extremely significant, and the content difference of metabolites including L-asparagine, L-glutamic and γ-aminobutyric acid in the second-grade Huang Guanyin oolong tea from two areas were extremely significant. In this study, the differential metabolites associated with the quality of Yunxiao and Wuyi Huang Guanyin oolong teas were screened out based on the widely targeted metabolomics analysis, providing a reference for elucidating the quality characteristics of Huang Guanyin oolong teas from the two areas.
