Abstract:A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was established for the simultaneous determination of cyazofamid and its major metabolite 4-chloro-5-(4-methylphenyl )-1H-imidazol-2-nitrile (CCIM) to evaluate their safety in fruits. The sample was extracted with acetonitrile containing 1.0% acetate acid and purified with octadecylsilane bonded phase (C18) powder and Florisil powder. Then the UPLC separation of sample was performed on Capcell PAK C18 column (150 mm×2.0 mm, 5 μm) and determined with multiple reaction monitoring mode (MRM) through HPLC-MS/MS. The results showed good linear relationships between the peak areas and the mass concentrations for both cyazofamid and CCIM in the range of 0.001~0.040 μg/mL (R≥0.999 3). When the spiked levels were between 0.004~0.080 mg/kg, the recoveries of cyazofamid and CCIM were in the range of 80.71%~103.27% and 76.62%~100.20%, respectively, and the relative standard deviations were between 4.14%~11.15% and 3.24%~11.16%, respectively. The LODs and LOQs of cyazofamid and CCIM in fruits were between 0.001 5~0.003 8 mg/kg and 0.003 0~0.007 9 mg/kg, respectively. The method was simple, rapid, high sensitivity and accurate, which could satisfy the rapid qualitative and quantitative detection of cyazofamid and CCIM residues in fruit samples.
