Immobilized Pyridoxine Kinase Catalyzes Synthesis of Pyridoxine Phosphate

doi:10.12441/spyswjs.20221220001

Home > Archive>Volume 43, Issue 12, 2024 >119-126. DOI:10.12441/spyswjs.20221220001

Immobilized Pyridoxine Kinase Catalyzes Synthesis of Pyridoxine Phosphate
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                        10.12441/spyswjs.20221220001
                    
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Affiliation:1.School of Pharmacy, Jiangsu Ocean University, Lianyungang 222005, China;2.Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Jiangsu Ocean University, Lianyungang 222005, China
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[Objective] According to the principle of specific binding of nickel-chelated agarose affinity chromatography medium (Ni-NTA) to histidine (His)-tagged proteins, the histidine-tagged Escherichia coli (E. coli)-derived pyridoxine kinase (EcpdxK) was immobilized with the Ni-NTA to form an immobilized reactor, and the optimal reaction conditions for the conversion of pyridoxine (PN) to pyridoxine phosphate (PNP) were investigated. [Method] The effects of temperature, pH, metal ion concentration, and recycling times on this catalytic reaction were investigated by one-way analysis of variance. [Result] The optimal reaction conditions of the immobilized EcpdxK were 55 ℃, pH 5.0, and 30 mmol/L Mg²⁺, under which the immobilized EcpdxK was able to convert 99% of PN into PNP within 10 min. In the immobilized EcpdxK-packed bed reactor, the column retention time of the EcpdxK reaction liquid was 10 min, and the conversion efficiency was still as high as 93% after 12 h of continuous reaction. [Conclusion] The immobilized EcpdxK has good temperature tolerance, pH tolerance, and operational stability, and the immobilized EcpdxK-catalyzed synthesis of PNP is a simple and cost-effective process with the potential for industrial application.

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HOU Xuewen, LI Menglei, YE Lei, QIU Min, ZHANG Kunxiao. Immobilized Pyridoxine Kinase Catalyzes Synthesis of Pyridoxine Phosphate[J]. Journal of Food Science and Biotechnology,2024,43(12):119-126.

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Received:December 20,2022
Revised:January 09,2023
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Online: April 11,2025
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