Abstract:Dog chews is a kind of snack for pet with oral cleaning effects. This research studied the effect of adding tea or tea polyphenols in dog chews on oral deodorization and dental care using in vitro simulation and animal experiments. The effect of Oolong tea, black tea and Pu'er tea on removing hydrogen sulfide was determined in vitro, which showed that Oolong tea had the best deodorization efficiency. The dog chews with the Oolong tea mass fraction of 0.5%~2.0% and tea polyphenols of 0.25%~0.75% were tested on dogs for 3 weeks. The results showed that the elimination rate of hydrogen sulfide in the oral of experimental dogs increased with the increase of the added effective substances. As the amount of Oolong tea was 2.0% or the mass fraction of tea polyphenols was 0.75%, the elimination rate of hydrogen sulfide in the oral cavity was close to 100%, and the tartar reduction rate was significantly increased to more than 60%. Fits of the total phenolics mass fraction and the effects showed that the elimination rate of hydrogen sulfide and the reduction rate of tartar both increased with the increasing of total phenolics mass fraction. Notably, there was a strong correlation between the total phenolics mass fraction and the elimination rate of hydrogen sulfide and the reduction rate of tartar. This study demonstrated the positive effect of polyphenols dog chews on dog oral health.