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Home > Archive>Volume 43, Issue 6, 2024 >58-66. DOI:10.12441/spyswjs.20230106002
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Efficient Production of 5-KetoFructose by Gluconobacter oxydans via Overexpression of Fructose Dehydrogenase
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    Abstract:

    5-Ketofructose(5-KF) has a similar sweet taste quality as D-fructose, and cannot be metabolized by humans, giving it a low caloric value. These properties illustrate 5-KF can become a potential candidate for a non-nutritive natural sweetener. In this study, the 5-KF producing strain was constructed via overexpression of membrane-bound fructose dehydrogenase gene from Gluconobacter japonicus in Gluconobacter oxydans. By screening different expression hosts, expression vectors and promoters, the engineered strain QT-10 which can efficiently synthesis 5-KF was obtained. The fed fermentation process was optimized in a 3 L fermenter. Finally, 650 g/L D-fructose could be completely transformed in 82 h with continuous fructose-feeding strategy, and up to 608 g/L 5-KF was reached with the yield of 94.6% and the space-time yield of 7.41 g/(L·h).

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QIAN Xue, ZHAO Chen-xiu, LIN Jin-ping, WEI Dong-zhi. Efficient Production of 5-KetoFructose by Gluconobacter oxydans via Overexpression of Fructose Dehydrogenase[J]. Journal of Food Science and Biotechnology,2024,43(6):58-66.

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  • Online: August 16,2024
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