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Home > Archive>Volume 43, Issue 2, 2024 >23-29. DOI:10.12441/spyswjs.20230109001
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Screening of Lyoprotectants and Optimization of High-Density Freeze-Drying Process for Bifidobacterium adolescentis
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    Abstract:

    To investigate the most suitable lyophilization protection formula for Bifidobacterium adolescentis and improve the efficiency of industrial production, the authors examined the lyophilization protection properties of various saccharide and protein protectants for B. adolescentis, the protection effect of compounding saccharide protectants with different structures and the protective effect of that with protein. Finally, the ratio between the dried mush and the lyophilizing agent was optimized to achieve high-density lyophilization. The results showed that the protective effect of saccharide (alcohol) protectants on B. adolescentis was generally higher than that of protein lyophilization protectants, and the lyophilization protection effect of small molecule saccharide below tetrasaccharides was better, among which sorbitol and raffinose had the best effect, and the lyophilization survival rate increased to about 56% after the combination at the mass ratio of 1∶1. The lyophilization pr

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PAN Zi-yi, CHEN Ze-hua, MAO Bing-yong, TANG Xin, NING Chu-guang, LI Wen-zhi, CUI Shu-mao, ZHAO Jian-xin, CHEN Wei. Screening of Lyoprotectants and Optimization of High-Density Freeze-Drying Process for Bifidobacterium adolescentis[J]. Journal of Food Science and Biotechnology,2024,43(2):23-29.

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  • Online: March 15,2024
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