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Home > Archive>Volume 44, Issue 8, 2025 >139-147. DOI:10.12441/spyswjs.20240103001
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Co-expression and Application of Enzymes for Preparation of β-Nicotinamide Mononucleotide
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1.School of Biotechnology, Jiangnan University, Wuxi 214122, China;2.Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;3.State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China

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    Abstract:

    [Objective] This study aims to reduce the cost and simplify the production process of β-nicotinamide mononucleotide (β-NMN) by the enzyme catalytic method. [Method] On the basis of the previously established β-NMN synthesis system with six enzymes, the six enzyme genes were combined in pairs to construct double-enzyme co-expression recombinant strains. Each crude enzyme solution obtained by co-expression was used for multi-enzyme catalytic cascade reaction to synthesize β-NMN. [Result] The yield of β-NMN was as high as 72.90% when the concentration of the substrate D-ribose was 10 mmol/L. The optimal temperature and IPTG induction concentration for inducing the enzyme expression of the recombinant strains were 25 ℃ and 0 mmol/L, respectively. Finally, the salt ions and polyhydroxy compounds that significantly improved the storage stability of 5-phosphate ribose-1-pyrophosphate synthase (EcPrs) were identified. Mn2+, lactose, and sorbitol basically had no effect on the yield of β-NMN. [Conclusion] The multi-enzyme catalytic cascade reaction for the preparation of β-NMN under dual-enzyme co-expression system and the optimization for EcPrs storage stability provide a new idea for the enzyme catalytic synthesis of β-NMN.

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LIU Jiehu, SU Lingqia, FU Huixian, WU Jing. Co-expression and Application of Enzymes for Preparation of β-Nicotinamide Mononucleotide[J]. Journal of Food Science and Biotechnology,2025,44(8):139-147.

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History
  • Received:January 03,2024
  • Revised:March 09,2024
  • Adopted:
  • Online: November 26,2025
  • Published:
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