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Home > Archive>Volume 44, Issue 12, 2025 >18-29. DOI:10.12441/spyswjs.20250312001
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Construction and Optimization of an Efficient Tyrosol Biosynthesis System in Escherichia coli
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1School of Biotechnology, Jiangnan University, Wuxi 214122, China;2Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China

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    Abstract:

    [Objective] An efficient biosynthetic system was constructed in Escherichia coli to achieve high-yield production of tyramine and its key derivative tyrosol.[Method] Through the analysis of protein expression, enzymatic properties, and whole-cell catalysis results, tyrosine decarboxylase (TDC) derived from Enterococcus faecalis was selected as the key enzyme for tyramine synthesis. Subsequently, tyramine oxidase (TYO) was introduced to construct the TDC-TYO pathway for tyrosol production. Meanwhile, the LAAD-PDC pathway for tyrosol synthesis was established. A dual-enzyme co-expression system was established, and the effects of tandem enzyme order, production pathways, and key enzyme sources on tyrosol yield were analyzed. The recombinant strain B1, carrying the plasmid pET28a-EfTDC-TYO, was obtained through whole-cell catalysis, and the reaction conditions were optimized.[Result] Under optimal reaction parameters, the recombinant strain B1 achieved a tyrosol yield of 3.96 g/L within 24 h, with a substrate conversion rate of 51.9%.[Conclusion] This study provides valuable insights for the biosynthesis of tyrosol and its derivatives.

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XIONG Yizhe, WANG Shougang, RAO Zhiming, XU Meijuan. Construction and Optimization of an Efficient Tyrosol Biosynthesis System in Escherichia coli[J]. Journal of Food Science and Biotechnology,2025,44(12):18-29.

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History
  • Received:March 12,2025
  • Revised:April 30,2025
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  • Online: March 02,2026
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