Abstract:Optimizing ingredient of hybridization solution and concentration of probes was conducted. 5'-biotinylated PCR product was automatically hybridized to a digoxigenin-labeled probe presented in the PCR reaction. The hybridization is performed as part of the

Abstract:The ice nucleation activity and the anti-leaking capability are both important technical parameters in INA bacteria immobilization which can be used for freezing concentration of food. Both co-immobilization and direct immobilization by alginate gel are g

Abstract:A L-Arginine producer strain YDM403(His , SGr, D-Argr, methyl-Cycr) was selected out by using D-Arginine and Methy-Cysteineas resistants after stepwise mutagenic treatment with N-methyl-N'-nitrosoguanidine(NTG)from Corynebacterium crenatum JND28(His-, SGr

Abstract:对兽疫链球菌NUF-035进行紫外诱变,得到突变菌株NUF-036,使透明质酸的产量从1.8 g/L提高到3.0 g/L,相对分子质量从2.1×10~6提高到3.2×10~6,突变株经多次传代,透明质酸产量及相对分子质量保持稳定.

Abstract:This paper compared the methods of zingibain extracting by alcohol, tannin, and ultra fil-tration (UF). It showed that the zirigibain extracted by UF had the highest proteolytic activity and the maximum permeate flux when operating pressure was kept at 0.

Abstract:将大连蛇岛蝮蛇毒类凝血酶(Gloshedobin)基因克隆到载体pET32-a(+)上,在T7lac启动子下以N端融合硫氧还蛋白的形式在大肠杆菌BL21(DE3)中进行表达,诱导条件为:30℃诱导6 h,IPTG浓度为1 mmol/L.利用固定化金属离子(Ni2+)亲和层析分别对胞内可溶物和变性条件下的包涵体进行纯化.通过SDS-PAGE检测融合蛋白的纯度,采用点杂交和纤维蛋白凝结活性检测分析,显示纯化产物具有较高生物活性.

Abstract:Pichia stipitis Xylitol dehydrogenase (XDH) Gene XYL2 was amplified by PCR. The XYL2 gene were placed under the triose phosphate isomerase (TPI) promoter of yeast vector pYX212 to produce the fusion expression vector pYX-XYL2. pYX-XYL2 was transformed int

Abstract:A strain Trichoderma viride ZC producing extracellular neutral cellulase was screened. The effect of various carbon source, mixed carbon source, and ratio of corn fibre to wheat bran on enzyme activity in shaking flasks was investigated. Fermentation cult

Abstract:用Brevibacterium sp.DGCDC-82在7L发酵罐中分批培养.分别对添加剂吐温-80、培养温度、培养基的pH、通风量和搅拌速度在胆固醇氧化酶的生产中的影响进行了研究.结果显示以上条件均对产酶有影响.在不同的发酵阶段改变发酵操作条件,发酵20 h最高酶活可达1203U/L,生产强度可达60 U/(L·h).既有效地提高了胆固醇氧化酶的产量,又防止了发酵过程中的泡沫外溢.

Abstract:In this paper, a metabolic network of the Corynebacterium glutamicum NS611 was estab-lished and modified. It showed that glucose was mainly metabolized through the EMP pathway. In the production phase, the metabolic flux of the pyruvate carboxylation was

Abstract:Experimental models were constructed using neural network to train experimental data, and the predicted values of the models were compared with the measured values. The result showed that theses models can accurately predict the time course of cell growth

Abstract:The effect of Zn on growth and metabolism in Agaricus blazei Murill was studied. The Zn-resisting and Zn-enriching ability of Agaricus blazei Murill, as well as the effect of Zn on metabolism of polysaccharide and amino acids were examined. The results sh

Abstract:The operation of nitrogen removal from brewery wastewater by the combined process of UASB + A2O was studied in this paper. It was shown that the nitrogen removal efficiency of 70% -98% could be achieved by keeping C/N above 15.20 Via introducing part of r

Abstract:利用紫外分光光度法研究并确定了从发酵菌体中提取辅酶Q10的工艺条件,即90℃回流30 min和石油醚萃取等,结果表明,采用皂化法提取、紫外分光光度法测定发酵菌体中辅酶Q10含量,不但具有较好的重现性和稳定性,而且其平均回收率高达(101.36±3.4)％,RSD为3.1％(n=3).

Abstract:对准纳米银对番茄汁、番茄-胡萝卜混合汁等蔬菜汁的防腐功效及准纳米银与其它防腐剂的复合防腐效果进行了研究.结果表明,当准纳米银溶液单独用于番茄汁作为抗菌防腐剂时,其有效质量浓度为0.1 mg/L;当准纳米银溶液单独用于番茄-胡萝卜混合汁防腐时,0.1 mg/L的质量浓度同样可以起到较好的保鲜效果,但是必须给予适度的热力杀菌;当番茄汁采用准纳米银-尼泊金乙酯复合防腐剂处理时,其最佳组合为:准纳米银0.04 mg/L,尼泊金乙酯200mg/L;而这种复合处理用于番茄-胡萝卜混合汁时,则需要在95～100℃条件下杀菌5 min.

Abstract:为提高莪术挥发油生物利用度,研究了β-环糊精包结莪术油的技术参数.以包合物油包结率及包合物收得率为考察指标,运用饱和水溶液包封法进行包结.当V(油):m(β-环糊精):V(水)=1 mL:4 g:60 mL,搅拌时间为2 h时,包合效果最佳,可稳定保持平均油包结率为62.29％.电镜观察证明了包合物的形成,GC-MS色谱分析表明包合前后莪术挥发油的化学组成基本不变.

Abstract:The methods of detecting the nucleic acid content, extracting the nucleic acid from pro-tamine in sea fish processing, determinenating product purity, and identifing constituent have been studied in this paper. In the course of samples pro-processing, the

Abstract:利用碱提酸沉淀法从灰树花(Grifola frondosa)发酵菌丝体中获得碱提水不溶性多糖GAP,氯磺酸-吡啶法对GAP进行硫酸化修饰后,即制得能溶于水的硫酸化灰树花多糖.通过DEAE-Sepharose F.F.离子交换柱层析精制为一种相对分子量为2.8×10~4,硫酸基质量分数为16.4％的硫酸酯化灰树花多糖(S-GAP).琼脂糖电泳显示,S-GAP可被甲苯胺蓝染色且着色较深.S-GAP的紫外光谱和红外光谱分析表明,在波长208,264,268,286 nm以及波数1 230 cm-1、810 cm-1处有硫酸酯键的特征吸收峰.

Abstract:The effect of a stabilizer and two kinds of emulsifiers at different levels on the quality of low fat ice cream was studied in this paper. The optimum amount of the stabilizer and emulsifiers was ob-tained by measuring rheological properties, destabilizat

Abstract:In this study, soy protein hydrolysate (SPH), maltodextrin (MD), emulsifier and hydrocolloids were used as the compound wall material, and powders of soybean salad oil and lard were produced by spray-drying technique. These products are very convenient fo

Abstract:The comparison between the oxidation of starch by sodium hypochlorite and kalium hyper-sulphate was reported in this paper . The results showed that sodium hypochlorite was better than kalium hypersulphate as the oxidative catalyst for preparing granular

Abstract:羊栖菜中分离得到的褐藻酸钠溶液的流变性质和胶凝性质研究结果表明:褐藻酸钠溶液粘度随剪切速率的增加而降低,是假塑性流体,其流动特性符合power-law模型,稠度指数为0.918Pa·sn,流动指数为0.8312,此外,温度、pH值、溶液质量分数也会影响褐藻酸钠溶液的粘度.褐藻酸钠溶液中加入Ca2+后形成褐藻酸钙凝胶,其凝胶强度随Ca2+添加量和褐藻酸钠溶液质量分数的增加而增加;凝胶弹性随Ca2+的添加量和褐藻酸钠溶液质量分数的增加而减少.

Abstract:Two colorimetric procedure for detecting flavonoids in Fagopyrum tataricum were evaluat-ed, by comparing with the results by HPLC. The results showed that AlCl3 spectrophotometric methods is superior to NaNO2-Al(NO3)3 methods both in detecting accuracy an

Abstract:用差示扫描量热法(DSC)测定了VC的熔点、热分解点,研究了热分解动力学.结果表明:VC熔点为193.457℃.融化热焓为258.73 J/g;VC分解的起始温度为221.620℃,分解热焓为-58.768 J/g;计算得到其热分解动力学参数Ea=1710.067±239.309 kJ/mol,并据此推算出在等温条件下反映热稳定性的分解率、时间、温度之间的关系.

Abstract:This paper investigated the status of fruits and vegetables' production, circulation and stor-age in China within the recent years; the authors also pointed out that though the output of fruits and vegetables in China is the greatest in the world, it is i