Abstract:This study systematically compared the effect of high concentration (10~5 000 mg/L) and low concentration(2.5~50.0 mg/L) of calcium lignosulfonate on the yield of biomass and three high-value products by using mixotrophic Phaeodactylum tricornutum growth in shake flasks with an aim to determine the optimal dose and effect. The results showed that at the optimal concentration of calcium lignosulfonate (10 mg/L), the biomass yield reached to 281.32 mg/(L·d). Additionally, the yields of fucoxanthin(Fx), eicosapentaenoic acid(EPA) and chrysolaminarin(Chrl) were 4.41, 10.60 and 57.24 mg/(L·d), respectively, which were 9.98%, 1.92%, and 7.27% higher than that in the control group. Notably, the yields of Fx and Chrl significantly increased (P<0.05). The author developed a novel mixotrophic culture model with the addition of calcium lignosulfonate, which was able to achieve a simultaneous yield enhancement of the three high-value products in mixotrophic Phaeodactylum tricornutum, providing a new technical support for improving the large-scale cultivation efficiency and application value of Phaeodactylum tricornutum.
