Abstract:Aflatoxins are a group of mycotoxins with strong mutagenic and carcinogenic properties.Various commodities including crop and feed materials are easy to be contaminated with aflatoxin.Saccharomyces cerevisiae have been reported to bind or degrade aflatoxin.Here,detoxification of aflatoxin B1(AFB1) by wild-type strain of S.cerevisiae(BY4742) and three mutants of anti-oxidative relating genes(zwf1Δ,sod2Δ and glr1Δ) were determined by HPLC.In PBS buffer,AFB1 binding abilities of viable and dead cells were 74%-76% and 71%-73%,respectively,indicating AFB1 was removed by yeast cells mainly through cell adsorption.In YPD medium,clearance of AFB1 by three mutant viable cells reduced,while that by wild-type BY4742 remaining high.AFB1 binding ability of glr1Δ decreased most seriously,then was that of sod2Δ and zwf1Δ.Thus,the deletion of critical anti-oxidative relating genes would decrease the AFB1 binding ability of S.cerevisiae growing cells.
