黑加仑花色苷抗禽白血病A亚群病毒作用

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黑加仑花色苷抗禽白血病A亚群病毒作用
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基金项目:国家自然科学基金项目(30972048);北京市自然科学基金重点项目(6111001)

Study on Anti-Avian Leukosis Virus Subgroup A Activity of Anthocyanin from A Blackcurrant

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研究了黑加仑花色苷(Anthocyanin from Blackcurrant,ACB)对禽白血病A亚群病毒(Avian leukosis virus subgroup A,ALV-A)的抑制作用。作者以ACB粉为原料,采用高效液相色谱仪与质谱仪联机(HPLC-MS)的方法鉴定了其主要活性成分,随后采用体外实验,应用MTT法和观察细胞形态法检测了ACB对鸡成纤维细胞系(DF1)的细胞毒性的影响,建立细胞模型,研究加入ACB后对ALV-A的预防和治疗作用。结果表明:ACB主要含飞燕草色素-3-鼠李糖苷-5-葡萄糖苷和矢车菊色素-3-鼠李糖苷-5-葡萄糖苷两种组分;ACB对DF1无细胞毒性的质量浓度为10μg/mL;基于ACB的预防和治疗实验,ACB可明显抑制ALV-A的增殖,具有良好的剂量-效应关系,因此ACB具有一定的体外抗ALV-A作用。

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Inhibitory action of anthocyanin from blackcurrant(ACB) to avian leukosis virus subgroup A(ALV-A) were studied.ACB powder was chosen as the raw material and its anthocyanins structure was identified by HPLC-MS.The morphology feature and survival rate of cells treated with anthocyanin were determinated by using MTT method and microscope.Furthermore,establishing the cell modle of anthocyanins inhibiting ALV-A which infecting in DF-1 cells,and then researched ACB’s prevention and treatment roles on the cells which infected with ALV-A.The results showed that ACB contained two main components as delphinidin-3-rhamnoside-5-glucoside and cyaniding-3-rhamnoside-5-glucoside.The cells grew well that treated with anthocyanin under the concentration of 10 μg/mL.There was a significantly inhibitory effect on restraining the proliferation of ALV-A after treating with anthocyanins.It was concluded that ACB had an inhibitory effect on ALV-A in vitro.

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雷用东,王丹,童军茂,张莉,马越,张超,赵晓燕.黑加仑花色苷抗禽白血病A亚群病毒作用[J].食品与生物技术学报,2013,32(8):821-827.

LEI Yong-dong, WANG Dan, TONG Jun-mao, ZHANG Li, MA Yue, ZHANG Chao, ZHAO Xiao-yan. Study on Anti-Avian Leukosis Virus Subgroup A Activity of Anthocyanin from A Blackcurrant[J]. Journal of Food Science and Biotechnology,2013,32(8):821-827.

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收稿日期:2012-11-24
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在线发布日期: 2013-10-28
出版日期: 2013-08-25

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