微波辅助提取和田玉枣环磷酸腺苷的工艺及与其他方法比较

doi:10.3969/j.issn.1673-1689.2014.03.011

首页 > 过刊浏览>2014年第33卷第3期 >293-300. DOI:10.3969/j.issn.1673-1689.2014.03.011

微波辅助提取和田玉枣环磷酸腺苷的工艺及与其他方法比较
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                        10.3969/j.issn.1673-1689.2014.03.011
                    
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基金项目:科技部农业科技成果转化基金项目(2011GB2360);陕西省科技计划项目(2012JM3014);陕西省教育厅科研计划项目(12JK0818);陕西学前师范学院科研基金项目(2013KJ063)

Processing Parameters for Extraction of cAMP from Hetian Jade Jujuba by Microwave-Assisted Extraction and Comparison of Different Extracting Methods

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研究了微波辅助提取和田玉枣环磷酸腺苷(cAMP)工艺参数,并与传统溶剂法和超声波辅助法对比。结果表明,微波辅助提取和田玉枣cAMP最优工艺条件为:乙醇体积分数10%,微波功率300 W、微波时间3 min、浸泡时间6 h、料液比1∶30,和田玉枣cAMP提取量为795.63μg/g;各因素影响和田玉枣cAMP提取量的显著顺序:微波时间>微波功率>料液比>浸泡时间;微波辅助法较传统溶剂法和超声波辅助法提取量高、提取时间短、能耗低。采用LS-200型树脂分离纯化和田玉枣cAMP的分离效率为97.81%,cAMP粗品纯度3.47%。

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The process parameters for the microwave-assisted extraction of cAMP from Hetianyuzao systemically was investigated in the study,and the results were compared with the traditional solvent extraction and ultrasonic-assisted extraction methods.The processing parameters of extracting cAMP from Hetian jade jujube with microwave-assisted extraction were obtained and listed as follows that:ethanol concentration 10%,microwave-assisted power 300 W,microwave-assisted extraction time3min,soak period 6 h,liquid/solid ratio 1 ∶30;Under the optimal extraction conditions,the yield of cAMP was 795.63 μg/(g·dw). The effect sequence of four main factors for the yield were as follows: microwave time>microwave power>liquid/solid ratio>soak period time. Microwave-assisted method extracted more cAMP than that of the traditional solvent extraction during short time,and extracted more cAMP than ultrasonic wave-assisted extraction with low energy consumption. The separation efficiency of cAMP by LS-200 was 97.81%,in 3.47% purity.

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王立霞.微波辅助提取和田玉枣环磷酸腺苷的工艺及与其他方法比较[J].食品与生物技术学报,2014,33(3):293-300.

WANG Lixia. Processing Parameters for Extraction of cAMP from Hetian Jade Jujuba by Microwave-Assisted Extraction and Comparison of Different Extracting Methods[J]. Journal of Food Science and Biotechnology,2014,33(3):293-300.

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在线发布日期: 2014-06-16
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