基于RNA-Seq的油茶种子油脂合成过程磷脂酰肌醇信号系统的基因分析

doi:10.3969/j.issn.1673-1689.2014.06.004

首页 > 过刊浏览>2014年第33卷第6期 >583-589. DOI:10.3969/j.issn.1673-1689.2014.06.004

基于RNA-Seq的油茶种子油脂合成过程磷脂酰肌醇信号系统的基因分析
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                        10.3969/j.issn.1673-1689.2014.06.004
                    
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Gene Analysis of Phosphatidylinositol Signaling System in Fatty Acide Developing of Camellia oleifera Seeds Based on RNA-Seq

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摘要:

磷脂酰肌醇信号系统在植物的种子萌发、生长发育、繁殖、衰老及对环境因素的应激反应中具有重要的作用。在构建油茶种子转录组数据库，以及油茶种子果实膨大期和油脂合成高峰期的表达谱数据库的基础上，对油茶种子磷脂酰肌醇信号系统的相关基因进行了分析。转录组数据分析结果表明，油茶种子磷脂酰肌醇信号系统非冗余基因Unigene序列共212条，包含PIK、PIPK、PIS、DGK、PLC等12种关键基因；表达谱数据分析显示，油茶种子果实膨大期和油脂合成高峰期调控磷脂酰肌醇信号系统的关键基因具有不同的表达模式。克隆、调控这些关键基因将有利于研究油茶种子发育过程中磷脂酰肌醇信号途径与其他信号途径的相互作用，为提高油脂含量、培育高品质油脂的油茶育种提供依据。

Abstract:

Phosphatidylinositol(PI) signaling system was critical in many plant developmental processes including seed development, growth and reproduction, aging and cell responses to environmental stress. Based on the construction of the transcriptome library and the expression profile of Camellia oleifera seeds both in the fruit expanding period and at the peak stage of lipid biosynthesis and oil synthesis, the key enzyme genes related to PI signaling system has been analyzed in detail. The research suggested that there are 212 non-redundant gene unigenes related to PI signaling sistem of Camellia oleifera seeds which mainly encode 12 kind of key enzyme proteins such as PIK, PIPK, PIS, DGK, PLC et al. The results of expression profile analysis showed that these genes have different expression patterns in different developmental stage of Camellia oleifera seeds. The findings will be in favor of the research of interaction between IP pathway and other signaling pathways and provide a scientific basis for Camellia oleifera breeding of good quality.

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江南,谭晓风,张琳.基于RNA-Seq的油茶种子油脂合成过程磷脂酰肌醇信号系统的基因分析[J].食品与生物技术学报,2014,33(6):583-589.

JIANG Nan, TAN Xiaofeng, ZHANG Lin. Gene Analysis of Phosphatidylinositol Signaling System in Fatty Acide Developing of Camellia oleifera Seeds Based on RNA-Seq[J]. Journal of Food Science and Biotechnology,2014,33(6):583-589.

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在线发布日期: 2014-10-19
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